To evaluate the antiasthmatic and antianaphylactic activity of stem bark of Machilus macrantha in various experimental models. The antiasthmatic and antianaphylactic activity of bark of Machilus macrantha was studied on the histamine and acetylcholine (Ach) induced bronchospasm in guinea pigs, active and passive anaphylaxis in rats and mast cell degranulation induced by clonidine. Treatment with MMUSM (unsaponifiable matter from pet ether extract) and MMAlK treated groups resulted significantly protection (P<0.001) against acetylcholine and histamine aerosol-induced bronchospasm in guinea pigs. While treatment in active and passive sensitized rats with USM and alkaloid from stem bark of Machilus macrantha dose dependent level (at 50,100 mg/kg p.o) showed significantly reduction in number of disrupted mast cells (P<0.001) in a intestinal mesenteric specimen of rats when challenged with antigen (horse serum). It also significant reduced IgE levels (P<0.001) in animal compared to untreated control and standard (prednisolone) and also showed significant reduction in blood eosinophil count in actively sensitized rats. It also significant protects the mast cell disruption induced by clonidine (ex-vitro).USM and alkaloid from stem bark of Machilus macrantha exhibited non selective anti-histaminic, anti-cholinergic and bronchodilator activity may be due to mast cell stabilizing potential, suppression of IgE antibody production, eosinophil count and also inhibition of antigen induced histamine release. It may be due to some phytosterol like β-sitosterol, stigmasterol present in USM and alkaloid present in bark.
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